分子量：
這些預染蛋白質分子量標準是設計用於SDS-聚丙烯酰胺凝膠電泳中監測蛋白質的分離，對膜（PVDF，尼龍或硝化纖維素）Western轉移效率的驗證和對蛋白質的近似大小。
梯子aresupplied在凝膠緩衝液，並準備使用。不要加熱，稀釋，加裝貨前還原劑。
品質管制
經測試，在SDS聚丙烯酰胺凝膠電泳和Western印跡。
存儲緩衝區
20mM磷酸氫二鈉pH 8.0的（在25℃），2％SDS，0.2％的Triton X-100,10毫摩爾2-巰基乙醇，6M尿素和27％（V / V）甘油。
存儲
儲存在-20℃下12個月。
穩定在+ 4℃下3個月。
加載建議
1.無論是在室溫或37-40℃進行數分鐘以溶解沉澱出的固體1.Thaw的階梯。不煮沸。
2.Mix徹底，以確保該溶液是均勻的。
3.Load梯子的下列卷上SDS-聚丙烯酰胺凝膠：
-每孔5μl的用於微型凝膠，每孔3微升為印跡;
-每孔10微升對於大凝膠，每孔6微升的印跡
Applications
The BlueStar PLUS Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Quality Control
Under suggested conditions, BlueStar Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane.
Storage
Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long term storage, store at -20°C.
Loading
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
1.5~2.5 μl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.
All products are for research use only and not intended for human or animal diagnostic or therapeutic uses
The BlueStar PLUS Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Quality Control
Under suggested conditions, BlueStar PLUS Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane.
Storage
Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long term storage, store at -20°C.
Loading
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
1.5~2.5 μl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.
All products are for research use only and not intended for human or animal diagnostic or therapeutic uses
The BlueEasy Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (62,5 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 1 mM EDTA, and 33 % (v/v) Glycerol).
Quality Control
Under suggested conditions, BlueEasy Prestained Protein Ladder resolves 10 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose and other membranes.
Storage
Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long term storage, store at -20°C.
Loading
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
1.5~2.5 μl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.
|
Cat.No.: |
Product |
Content |
|
MWP03 |
BlueStar Prestained Protein Marker (500 µl) |
1x 500 µl Protein Marker in loading buffer, sufficient for 100 mini gels or 50 large gels |
|
MWP04 |
BlueStar PLUS Prestained Protein Marker (500 µl) |
1x 500 µl Protein Marker in loading buffer, sufficient for 100 mini gels or 50 large gels |
|
MWP06 |
BlueEasy Prestained Protein Marker (500 µl) |
1x 500 µl Protein Marker in loading buffer, sufficient for 100 mini gels or 50 large gels |
|
MWP05 |
JustBlue Prestained Protein Marker (500 µl) |
1x 500 µl Protein Marker in loading buffer, sufficient for 100 mini gels or 50 large gels |
|
MWP02 |
Pink Prestained Protein Marker (2 x 500 µl) |
2x 500 µl Protein Marker in loading buffer, sufficient for 100 mini gels or 50 large gels |
