The freezing medium BambankerTM offered by the Japanese company Lymphotec was developed initially just for their own R&D projects. They needed a suitable medium for long-term storage of highly sensitive cell lines, such as lymphocytes.
Thanks to the innovative formulation of the new freezing medium, an European Patent (EP 1347040) was granted and the development of this media was made commercially available. Today this innovative cell freezing medium BambankerTM is the market leader in Japan and characterized by many different published articles with very sensitive cell lines all over the world.
All common cell lines can be preserved resulting in a high number of intact cells after thawing. Recovery rates of even sensitive cells is much higher compared to regular media.
No dilution, no addition of components (like DMSO or glycerol). No programmable freezer and no sequential freezing steps are required - saves a lot of time. Shelf life is 24 months, when stored in a fridge (2 - 10 °C).
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Cultivation: |
15% FBS/DMEM (1 mM of Sodium pyruvate, 100 µM of NEAA, 100 µM of ß-ME, 1000 U/ml of LIF) was used as culture medium. Mouse Embryonic Fibroblasts (MEF) were used as „feeder cells“. |
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Freezing: |
Cells were frozen in 5 vials / ( 60mm dish corresponds to 3.0 x 106 cells/vial ). 1 ml/vial of BambankerTM freezing medium was added and the mixture was directly frozen in -80 °C. The following day, the vials were transferred to liquid nitrogen (slow freezing). |
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Thawing: |
Cells were incubated at 37 °C and transferred in cooled culture media. After collection, cells were seeded in 6 well plates and 6 cm dishes. |
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Results: |
Stabilization of Mouse Embryonic Stem Cells by using BambankerTM was succesful. Cells were undifferentiated, even after freeze and thaw procedure. No modifications of cells could be observed. |
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Cat.No.: |
Product |
Content |
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BB01 |
BambankerTM |
120 ml Freezing medium BambankerTM |
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BB02 |
BambankerTM |
5 x 20 ml Freezing medium BambankerTM |
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BB03 |
BambankerTM |
20 ml Freezing medium BambankerTM |
