The special buffer formulation permits a high RNA concentration. Other reverse transcriptases are not able to process such large quantities.
The FastGene® Scriptase Basic was designed for large RNA quantities, typically used in an endpoint RT-PCR. Nonetheless, it is also able to process lower RNA concentrations.
The FastGene® Scriptase Basic is available as enzyme only, containing the enzyme, buffer and dNTPs as well as a
cDNA synthesis kit which contains the components of the enzyme plus Oligo dTs, random hexamers and RNase inhibitor. We have also chosen a 100 reactions kit size so that you have enough for a whole 96-well plate.
(A) Competitor N (B) FastGene® Scriptase Basic
Fig. 1: Higher sensitivity of the FastGene® Scriptase Basic.
Compared to a wildtype MuLV (A) the FastGene® Scriptase Basic (B) is an optimized MuLV-Reverse transcriptase which has a higher sensitivity, being able to produce a template from RNA concentrations as low as 0.1 ng.
Cat. No. |
Product |
Content |
LS52 |
FastGene® Scriptase Basic (20.000 units at 200 U/μl) |
100 Reactions |
LS62 |
FastGene® Scriptase Basic cDNA Synthesis containing Oligo dTs, random hexamer and RNase inhibitor |
100 Reactions |